An Alkynylpyrimidine-Based Covalent Inhibitor That Targets a Unique Cysteine in NF-?B-Inducing Kinase

Raf Kinase Inhibitor Protein Interacts with NF- B-Inducing Kinase and TAK1 and Inhibits NF- B Activation

TNAP , a Novel Repressor of NF - B - inducing Kinase , Suppresses NF - B Activation

NFB-inducing kinase (NIK) has been implicated as an essential component of NFB activation. However, the regulatory mechanism of NIK signaling remains elusive. We have identified a novel NIK interacting protein, TNAP (for TRAFs and NIK-associated protein). In mammalian cells, TNAP physically interacts with NIK, TRAF2, and TRAF3 but not IKK1 or IKK2. TNAP specifically inhibits NFB activation indu...

NF- B-Inducing Kinase Establishes Self-Tolerance in a Thymic Stroma-Dependent Manner

Interleukin-1 Induction of NF B Is Partially Regulated by H2O2-mediated Activation of NF B-inducing Kinase*

Reactive oxygen species (ROS) have been demonstrated to act as second messengers in a number of signal transduction pathways, including NF B. However, the mechanism(s) by which ROS regulate NF B remain unclear and controversial. In the present report, we describe a mechanism whereby interleukin-1 (IL-1 ) stimulation of NF B is partially regulated by H2O2-mediated activation of NIK and subsequen...

Development of an insect-cell-based assay for detection of kinase inhibition using NF-kappaB-inducing kinase as a paradigm.

Identification of small-molecule inhibitors by high-throughput screening necessitates the development of robust, reproducible and cost-effective assays. The assay approach adopted may utilize isolated proteins or whole cells containing the target of interest. To enable protein-based assays, the baculovirus expression system is commonly used for generation and isolation of recombinant proteins. ...